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何水林

发布时间：2022年10月20日 10:51文章来源： 浏览次数：

一．个人简况

何水林，教授，博士生导师。福建省科技创新领军人才，福建省“百千万人才工程”培养对象，福建省蔬菜技术产业体系首席专家，福建农林大学“金山学者”创新人才，是PLos Genetics、 Plant Cell & Environment、Journal of Experiment Botany、 Frontier in Plant Science和Journal of Hazardous Materials等国际刊物的审稿专家，国家自然基金通讯评审专家。

近几年来，先后承担国家自然科学基金、国家转基因专项、福建省自然基金重大项目、重点项目以及蔬菜重大专项、种业工程重大专项等国家和省部级研究课题20多项。在辣椒抗青枯病及耐高温高湿的关联、高温高湿下抗病分子机制方面开展了较为系统的研究。近几年在国际著名刊物 New Phytologist, Plant Physiology, Journal of Experimental Botany, Plant Cell & Environment, Molecular Plant Pathology, Plant Cell and Physiology和 Molecular Plant-Microbe Interactions等发表论文40多篇。此外，还育成了闽椒系列和福农系列辣椒新品种10余个，通过省级认定或国家登记，并已经在省内外推广应用。

二. 研究领域和方向：

研究领域：

植物生物技术与分子生物学；蔬菜遗传改良与新品种选育，蔬菜设施在无公害栽培

主要研究方向：

1、蔬菜及水稻抗逆功能基因组学研究

（1）应答逆境逆境信号通路的分子剖析

植物抗病及抗逆是通过对病原菌侵染及逆境胁迫信号的感知、信号传导及相关基因表达的改变来实现的，受到复杂的信号传递网络的调控。本实验室长期以来围绕着植物应答逆境胁迫的信号传导网络的分子剖析，以茄科蔬菜辣椒为主要材料，瞄准辣椒在高温高湿下病害发生严重这一重要生产问题，着重研究辣椒应答疫霉及青枯菌等主要病原菌及高温高湿逆境的信号通路交互作用（crosstalk）中小G蛋白、激酶、转录因子（WRKY、NAC、ERF等）等重要节点的结构和功能，提出茄科植物应答高温高湿和抗病偶联的假说，发现了CaROP1、CaWRKY6和CaWRKY6等在辣椒协调应答病原菌侵染及高温高湿逆境过程中起重要的调节作用。

（2）辣椒与疫霉协同进化的分子机制研究

大量的研究发现植物在与病原菌协同进化过程中形成了PTI和ETI彼此交互作用的两个层次抗病机制，但这两个层次的抗病反应之间存在着信号通路和防御反应的重叠，暗示PTI和ETI之间存在着连续分布的中间态。我们以辣椒疫霉的系列非典型分子模式（PAMP）或非典型效应子（effector）为研究对象，发现由SRC2-1和SGT1等参与的辣椒应对elicitin的之字型协同进化关系。

2、辣椒、白萝卜遗传改良

以高温高湿下辣椒等作物抗病分子机制及相关分子标记开发研究为基础，开展分子标记、杂交育种、生态压力选择等相结合的辣椒等蔬菜高温高湿下抗病种质资源创新和新品种选育，已经选育出闽椒1号、闽椒2号、闽椒3号和闽椒4号以及金玉3号等蔬菜新品种经过省级认定，开展了其高产高效配套栽培技术研究，并在福建及江西等地示范推广应用。

3、蔬菜无公害生产关键技术研究和推广

本方向包括：

（1）诱导抗病小分子化合物筛选与在作物上应用，与美国加州大学河滨分校Thomas Eulgem教授实验室合作，利用对病原菌高效持续高效应答基因启动子与GUS报告基因的转基因植株，建立诱导抗病小分子化合物高通量筛选体系，目前已经筛选获得10种以上小分子化合物，其中DCA在茄科蔬菜和水果荔枝保鲜中表现出较大的应用潜力；

（2）蔬菜育苗基质研发，已经利用木屑等原料，研发出一种高效、廉价的蔬菜育苗基质；

（3）茄科蔬菜涝害恢复技术，通过应用抗病小分子化合物和生长调节剂，通过优化配方，研发出辣椒等茄科植物涝害淹水后的抗病恢复技术；

（4）研究了在闽东南沿海地区青花菜菜田的主要害虫发生规律，并建立了其可持续防治的技术体系。

三.承担的主要课题

1. 设施茄果类蔬菜育种攻关与产业化开发，“十四五”福建省种业创新与产业化工程 (2021—2025年)-农业良种育种攻关与产业化开发项目，zvcxnv2021008，2021.1-2022.12,1000.0万元。

2. 福建省现代蔬菜产业技术体系首席专家工作站－首席专家，闽农综[2019]144号，2019.10-2022.12；120.0万元。

3. 设施辣（甜）椒品种选育与产业化工程，“十三五”福建省种业创新与产业化工程项目重大专项，fjzycxny2017007，2017.1-2020.12；1000.0万元。

4. 辣椒含DWE的WRKY基因功能相关及其在协同应答高温及青枯菌中作用解析，国家自然基金，(31572136),2016-2019

5. 2.CaWRKY40介导辣椒耐高温高湿及抗青枯病分子机制解析，国家自然科学基金，31372061，2014-01-2017.12， 80万元。

6. 辣椒高温高湿下抗病功能基因组学研究及高产优质高抗辣椒设施型新品种选育与应用，福建省重大科技专项，66万元，2013.10-2016.12.

7. 抗病转基因水稻新品种培育，国家转基因生物新品种培育重大专项，2009ZX08001-015B,65万元，2009-2012

8. 激活水稻LURM基因表达化合物筛选及其诱导抗病活性分析，国家自然科学基金，30971718，2010-2012，30万元

9. CaWRKY5激活植物低温抗性的结构基础和分子机制研究，福建省自然科学基金重点项目，2008J0003，2008-2011，20万元

四. 近年来发表的部分代表性论文

  1. 1. Li, X., Weng, Y., Chen, Y., He, S., & Liu, Z. CaARP1/CaSGT1 module regulates vegetative growth and defense response of pepper plants against Phytophthora capsici. Plants, 2024.  

  2. 2. Wu, R., Wu, Z., Qing, Y., He, S., & Qiu, A. CaZingipain2 acts positively in pepper (Capsicum annuum L.) immunity against R. solanacearum. Plants, 2024.  

  3. 3. Yang, S., Wan, M., Cheng, X., Cheng, Q., & Shen, H. A 14-3-3 protein Ca16R acts positively in pepper immunity against Ralstonia solanacearum by interacting with CaASR1. Plants, 2024.  

  4. 4. Mou, S., Liu, Z., He, W., & He, S. Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a. Plant Physiology, 2024.  

  5. 5. Cheng, X., Wan, M., Song, Y., & He, S. CaSTH2 disables CaWRKY40 from activating pepper thermotolerance and immunity against Ralstonia solanacearum via physical interaction. Horticulture Research, 2024.  

  6. 6. Cai, W., Tao, Y., Cheng, X., & He, S. CaIAA2-CaARF9 module mediates the trade-off between pepper growth and immunity. Plant Biotechnology Journal, 2024.  

  7. 7. Huang, X., Yang, S., Zhang, Y., & He, S. Pepper mildew resistance locus O 1 (MLO1) context-specifically induces high-temperature-specific immunity and thermotolerance. Journal of Experimental Botany, 2023.  

  8. 8. Liu, K., Shi, L., Luo, H., He, S., & Liu, Z. Ralstonia solanacearum effector RipAK suppresses homodimerization of the host transcription factor ERF098 to enhance susceptibility and sensitivity of pepper plants to dehydration. The Plant Journal, 2023.  

  9. 9. Yang, S., Cai, W., Wu, R., & He, S. Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions. Nature Communications, 2023.  

  10. 10. Dang, F., Lin, J., Li, Y., He, S., & Wang, Y. SlWRKY30 and SlWRKY81 synergistically modulate tomato immunity to Ralstonia solanacearum by directly regulating [target genes]. Horticulture Research, 2023.  

  11. 11. Zhang, Y., Cai, W., Wang, A., & He, S. MADS-box protein AGL8 interacts with chromatin-remodeling component SWC4 to activate thermotolerance and environment-dependent immunity in pepper. Journal of Experimental Botany, 2023.  

  12. 12. Huang, Y., Cai, W., Lu, Q., Lv, J., Wan, M., Guan, D., Yang, S., & He, S. PMT6 is required for SWC4 in positively modulating pepper thermotolerance. International Journal of Molecular Sciences, 2023.  

  13. 13. Hussain, A., Liu, K., Ali, N., & He, S. CaASHH3 N-methyltransferase acts as a positive regulator of immunity against bacterial pathogens in pepper. International Journal of Molecular Sciences, 2022.  

  14. 14. Shi, L., Li, X., Weng, Y., Cai, H., Liu, K., Xie, B., Ansar, H., Guan, D., He, S., & Liu, Z. The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance. The Plant Journal, 2022, DOI: 10.1111/tpj.15790.  

  15. 15. Lu, Q., Huang, Y., Wang, H., & He, S. CabZIP23 integrates in CabZIP63–CaWRKY40 cascade and turns CabZIP63 on mounting pepper immunity against Ralstonia solanacearum via physical interaction. International Journal of Molecular Sciences, 2022.  

  16. 16. Cai, W., Yang, S., Wu, R., & He, S. CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper. PLoS Genetics, 2022.  

  17. 17. Yang, S., Cai, W., Shen, L., & He, S. A CaCDPK29-CaWRKY27b module promotes CaWRKY40-mediated thermotolerance and immunity to Ralstonia solanacearum in pepper. New Phytologist, 2022.  

  18. 18. Cheng, W., Lin, M., Chu, M., & He, S. Phytophthora capsici RNAi-based gene silencing of RXLR effectors protects plants against the oomycete pathogen. Molecular Plant-Microbe Interactions, 2022.  

  19. 19. Yang, S., Cai, W., Shen, L., & He, S. Solanaceous plants switch to cytokinin-mediated immunity against Ralstonia solanacearum under high temperature and high humidity. Plant, Cell & Environment, 2022.Shi L，Li X，Weng Y，Cai H，Liu K，Xie B，Ansar H，Guan D，He S，Liu Z，The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance. The Plant Journal, 2022,DOI: 10.1111/tpj.15790.

  20. 20. Yang S, Cai W, Shen L, Wu R, Cao J, Tang W, Lu Q, Huang Y, Guan D, He S: Solanaceous plants switch to cytokinin-mediated immunity against Ralstonia solanacearum under high temperature and high humidity. Plant, Cell & Environment 2022, 45(2):459-478.

  21. 21. Yang S, Cai W, Shen L, Cao J, Liu C, Hu J, Guan D, He S: A CaCDPK29-CaWRKY27b module promotes CaWRKY40-mediated thermotolerance and immunity to Ralstonia solanacearum in pepper. The New Phytologist 2022, 233(4):1843-1863.

  22. 22. Cai W, Yang S, Wu R, Zheng Y, He S, Shen L, Guan D, He S: CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper. PLoS Genetics 2022, 18(2):e1010023.

  23. 23. Liu ZQ, Shi LP, Yang S, Qiu SS, Ma XL, Cai JS, Guan DY, Wang ZH, He SL: A conserved double-W box in the promoter of CaWRKY40 mediates autoregulation during response to pathogen attack and heat stress in pepper. Molecular Plant Pathology 2021, 22(1):3-18.

  24. 24. Cai W, Yang S, Wu R, Cao J, Shen L, Guan D, Shuilin H: Pepper NAC-type transcription factor NAC2c balances the trade-off between growth and defense responses. Plant Physiology 2021, 186(4):2169-2189.

  25. 25. Huang J, Shen L, Yang S, Guan D, He S: CaASR1 promotes salicylic acid- but represses jasmonic acid-dependent signaling to enhance the resistance of Capsicum annuum to bacterial wilt by modulating CabZIP63. Journal of Experimental Botany, 2020, 71(20):6538-6554.

  26. 26. Dang F, Lin J, Chen Y, Li GX, Guan D, Zheng SJ, He S: A feedback loop between CaWRKY41 and H2O2 coordinates the response to Ralstonia solanacearum and excess cadmium in pepper. Journal of Experimental Botany, 2019, 70(5):1581-1595.

  27. 27. Qiu A, Lei Y, Yang S, Wu J, Li J, Bao B, Cai Y, Wang S, Lin J, Wang Y et al: CaC3H14 encoding a tandem CCCH zinc finger protein is directly targeted by CaWRKY40 and positively regulates the response of pepper to inoculation by Ralstonia solanacearum. Molecular Plant Pathology 2018, 19(10):2221-2235.

  28. 28. Cheng W, Xiao Z, Cai H, Wang C, Hu Y, Xiao Y, Zheng Y, Shen L, Yang S, Liu Z et al: A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection. Molecular Plant Pathology 2017, 18(8):1089-1100.

  29. 29. Shen L, Liu Z, Yang S, Yang T, Liang J, Wen J, Liu Y, Li J, Shi L, Tang Q et al: Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40. Journal of Experimental Botany, 2016, 67(8):2439-2451.

  30. 30. Liu ZQ, Qiu AL, Shi LP, Cai JS, Huang XY, Yang S, Wang B, Shen L, Huang MK, Mou SL et al: SRC2-1 is required in PcINF1-induced pepper immunity by acting as an interacting partner of PcINF1. Journal of Experimental Botany,  2015, 66(13):3683-3698.

  31. 31. Cai H, Yang S, Yan Y, Xiao Z, Cheng J, Wu J, Qiu A, Lai Y, Mou S, Guan D et al: CaWRKY6 transcriptionally activates CaWRKY40, regulates Ralstonia solanacearum resistance, and confers high-temperature and high-humidity tolerance in pepper. Journal of Experimental Botany,  2015, 66(11):3163-3174.

  32. 32. Wang Y, Dang F, Liu Z, Wang X, Eulgem T, Lai Y, Yu L, She J, Shi Y, Lin J et al: CaWRKY58, encoding a group I WRKY transcription factor of Capsicum annuum, negatively regulates resistance to Ralstonia solanacearum infection. Molecular Plant Pathology 2013, 14(2):131-144.

  33. 33. Dang FF, Wang YN, Yu L, Eulgem T, Lai Y, Liu ZQ, Wang X, Qiu AL, Zhang TX, Lin J et al: CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection. Plant, Cell & Environment 2013, 36(4):757-774.

五、育成品种

1. 闽椒1号，福建省非主要农作物新品种认定委员会认定，2010

2. 闽椒2号，福建省非主要农作物新品种认定委员会认定，2010

3. 金玉3号，福建省非主要农作物新品种认定委员会认定，2012

4. 闽椒3号，福建省非主要农作物新品种认定委员会认定，2016

5. 闽椒4号，福建省非主要农作物新品种认定委员会认定，2016

6. 福农1号，通过国家品种登记，2021

7. 福农2号，通过国家品种登记，2021

8. 福农4号，通过国家品种登记，2021

六、出版教材和专著

1. 植保素代谢与植物防御反应，广东科技出版社，2002年（独著）

2. 基因工程，科学出版社，2008年（主编）

3. 农业总论，中国农业大学出版社(面向二十一世纪教材)（参编）

七、联系方式

1. E-mail: shlhe201304@aliyun.com；469598131@qq.com

2. 联系电话： 18606998458





Vitae

Name:Shuilin HE

Unit:College of Crop Science, FujianAgriculture & ForestryUniversity

Title: Professor, Associated Dean

Profile:

Dr. He received his Bachelor degree in Agronomy from JiangxiAgricultureUniversity in 1988, his Master degree in Agronomy from HuazhongAgricultureUniversity in 1991 and then worked 4 years as assistant and 4 years as lecturer in FujianAgricultureUniversity. He worked from 1996 October to 1998 January in KyungpookNationalUniversity as a visiting scholar and received his Ph.D. in Agronomy from FujianAgricultureUniversity in 1998. After he got his Ph.D, he worked 4 years as associated professor in College of Crop Science in Fujian Agriculture University and moved to College of Life Science and was promoted to be associated dean of College of Life Science, He worked there as a full time professor in plant stress physiology focusing on pepper. In 2008, he visited Eulgem Thomas’ lab in University of California, Riverside for 4 months as visiting scholar. In 2011, he moved back to the College of Crop Science. Currently, Dr. He is associated dean of College  of Crop Science and the thesis advisor for Ph.D. students in functional genomics of plant stress physiology.

Research work:

Functional genomics of plant stress physiology

Publications:

  1. Shi L，Li X，Weng Y，Cai H，Liu K，Xie B，Ansar H，Guan D，He S，Liu Z，The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance. The Plant Journal, 2022,DOI: 10.1111/tpj.15790

  2. Yang S, Cai W, Shen L, Wu R, Cao J, Tang W, Lu Q, Huang Y, Guan D, He S: Solanaceous plants switch to cytokinin-mediated immunity against Ralstonia solanacearum under high temperature and high humidity. Plant, Cell & Environment 2022, 45(2):459-478.

  3. Yang S, Cai W, Shen L, Cao J, Liu C, Hu J, Guan D, He S: A CaCDPK29-CaWRKY27b module promotes CaWRKY40-mediated thermotolerance and immunity to Ralstonia solanacearum in pepper. The New Phytologist 2022, 233(4):1843-1863.

  4. Cai W, Yang S, Wu R, Zheng Y, He S, Shen L, Guan D, He S: CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper. PLoS Genetics 2022, 18(2):e1010023.

  5. Liu ZQ, Shi LP, Yang S, Qiu SS, Ma XL, Cai JS, Guan DY, Wang ZH, He SL: A conserved double-W box in the promoter of CaWRKY40 mediates autoregulation during response to pathogen attack and heat stress in pepper. Molecular Plant Pathology 2021, 22(1):3-18.

  6. Cai W, Yang S, Wu R, Cao J, Shen L, Guan D, Shuilin H: Pepper NAC-type transcription factor NAC2c balances the trade-off between growth and defense responses. Plant Physiology 2021, 186(4):2169-2189.

  7. Huang J, Shen L, Yang S, Guan D, He S: CaASR1 promotes salicylic acid- but represses jasmonic acid-dependent signaling to enhance the resistance of Capsicum annuum to bacterial wilt by modulating CabZIP63. Journal of Experimental Botany, 2020, 71(20):6538-6554.

  8. Dang F, Lin J, Chen Y, Li GX, Guan D, Zheng SJ, He S: A feedback loop between CaWRKY41 and H2O2 coordinates the response to Ralstonia solanacearum and excess cadmium in pepper. Journal of Experimental Botany, 2019, 70(5):1581-1595.

  9. Qiu A, Lei Y, Yang S, Wu J, Li J, Bao B, Cai Y, Wang S, Lin J, Wang Y et al: CaC3H14 encoding a tandem CCCH zinc finger protein is directly targeted by CaWRKY40 and positively regulates the response of pepper to inoculation by Ralstonia solanacearum. Molecular Plant Pathology 2018, 19(10):2221-2235.

  10. Cheng W, Xiao Z, Cai H, Wang C, Hu Y, Xiao Y, Zheng Y, Shen L, Yang S, Liu Z et al: A novel leucine-rich repeat protein, CaLRR51, acts as a positive regulator in the response of pepper to Ralstonia solanacearum infection. Molecular Plant Pathology 2017, 18(8):1089-1100.

  11. Shen L, Liu Z, Yang S, Yang T, Liang J, Wen J, Liu Y, Li J, Shi L, Tang Q et al: Pepper CabZIP63 acts as a positive regulator during Ralstonia solanacearum or high temperature-high humidity challenge in a positive feedback loop with CaWRKY40. Journal of Experimental Botany, 2016, 67(8):2439-2451.

  12. Liu ZQ, Qiu AL, Shi LP, Cai JS, Huang XY, Yang S, Wang B, Shen L, Huang MK, Mou SL et al: SRC2-1 is required in PcINF1-induced pepper immunity by acting as an interacting partner of PcINF1. Journal of Experimental Botany,  2015, 66(13):3683-3698.

  13. Cai H, Yang S, Yan Y, Xiao Z, Cheng J, Wu J, Qiu A, Lai Y, Mou S, Guan D et al: CaWRKY6 transcriptionally activates CaWRKY40, regulates Ralstonia solanacearum resistance, and confers high-temperature and high-humidity tolerance in pepper. Journal of Experimental Botany,  2015, 66(11):3163-3174.

  14. Wang Y, Dang F, Liu Z, Wang X, Eulgem T, Lai Y, Yu L, She J, Shi Y, Lin J et al: CaWRKY58, encoding a group I WRKY transcription factor of Capsicum annuum, negatively regulates resistance to Ralstonia solanacearum infection. Molecular Plant Pathology 2013, 14(2):131-144.

  15. Dang FF, Wang YN, Yu L, Eulgem T, Lai Y, Liu ZQ, Wang X, Qiu AL, Zhang TX, Lin J et al: CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection. Plant, Cell & Environment 2013, 36(4):757-774.

Contact:

  1. Phone:86-591- 83793159, Mobile phone: 86-18606998458

  2. E-mail:shlhe201304@aliyun.com




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